Contact an Account ManagerDetermination of urapidil and its metabolites in human serum and urine: Comparison of liquid-liquid and fully automated liquid-solid extraction
Summary
Type: HPLC
Application #: 104050
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: electrochemical, +1.0V versus Ag/AgCl
Conditions: eluent: 15% methanol in 20mM NaClO4 pH 2.0, adjusted with perchloric acid, 2min isocratic, then gradient with 1%/min increase in methanol percentage for 25min
flow rate 1.0ml/min
temperature: 40°C
Authors: Zech K
Source: J Chromatogr 353 (1986) 351 - 360
Details
Legend: Fig. 4. Chromatograms obtained by direct injection of 50µl human urine a)
before and b) after oral administration of 30mg of urapidil solution. Urine was
collected between 2 and 4h. Chromatogram b)